Difference between revisions of "Main Page/BPHS 4090/Optical Tweezers of Onions"
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| − | <h2>Preparation of onion epidermis for optical trapping experiments <ref | + | <h2>Preparation of onion epidermis for optical trapping experiments <ref> Diagrams from Peterson LR, CA Peterson, and LH Melville (2008) Teaching Plant Anatomy through Creative Laboratory Exercises. National Research Council of Canada. Page 17.</ref> </h2> |
<p>The common onion (<i>Allium cepa</i>) is often used to examine individual plant cells because of the ease of isolating sheets of cells that are one cell thick. The onion bulb can be sectioned into quarters or eighths. Then, the individual scale leaves can be separated.</p> | <p>The common onion (<i>Allium cepa</i>) is often used to examine individual plant cells because of the ease of isolating sheets of cells that are one cell thick. The onion bulb can be sectioned into quarters or eighths. Then, the individual scale leaves can be separated.</p> | ||
<p>The exposed concave surface can be scored with a sharp razor blade. With a very fine pair of forceps, pieces of the epidermis can be lifted (note the transparency of the peel). Before doing so, have a microscope slide ready with a drop of distilled water (or artificial pond water) so that the peel doesn’t become dehydrated. Unlike the photographs, a thin strip of Vaseline will be placed on the microscope slide in a rectangular shape slightly smaller in dimensions then the cover slip. Having placed the epidermal peel in the water inside the Vaseline ‘dike’, carefully (gently) place the coverslip on top, pressing to create a seal around the perimeter.</p> | <p>The exposed concave surface can be scored with a sharp razor blade. With a very fine pair of forceps, pieces of the epidermis can be lifted (note the transparency of the peel). Before doing so, have a microscope slide ready with a drop of distilled water (or artificial pond water) so that the peel doesn’t become dehydrated. Unlike the photographs, a thin strip of Vaseline will be placed on the microscope slide in a rectangular shape slightly smaller in dimensions then the cover slip. Having placed the epidermal peel in the water inside the Vaseline ‘dike’, carefully (gently) place the coverslip on top, pressing to create a seal around the perimeter.</p> | ||
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[[http://multimedia.mcb.harvard.edu/anim_myosin.html]] | [[http://multimedia.mcb.harvard.edu/anim_myosin.html]] | ||
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<h2>References</h2> | <h2>References</h2> | ||
</references> | </references> | ||
Revision as of 10:36, 29 June 2010
Optical Tweezers of Onion Cells
Preparation of onion epidermis for optical trapping experiments [1]
The common onion (Allium cepa) is often used to examine individual plant cells because of the ease of isolating sheets of cells that are one cell thick. The onion bulb can be sectioned into quarters or eighths. Then, the individual scale leaves can be separated.
The exposed concave surface can be scored with a sharp razor blade. With a very fine pair of forceps, pieces of the epidermis can be lifted (note the transparency of the peel). Before doing so, have a microscope slide ready with a drop of distilled water (or artificial pond water) so that the peel doesn’t become dehydrated. Unlike the photographs, a thin strip of Vaseline will be placed on the microscope slide in a rectangular shape slightly smaller in dimensions then the cover slip. Having placed the epidermal peel in the water inside the Vaseline ‘dike’, carefully (gently) place the coverslip on top, pressing to create a seal around the perimeter.
Now ready to place in the holder on the optical bench (left), here is what the cells will look like (right, the nucleus and transvacuolar strands are indicated).
To view an animation on how the myosin motors move the spherosomes along the actin fibers: [[1]]
References
</references>
- ↑ Diagrams from Peterson LR, CA Peterson, and LH Melville (2008) Teaching Plant Anatomy through Creative Laboratory Exercises. National Research Council of Canada. Page 17.
