Main Page/BPHS 4090/microscopy I - Revision history

Rlew at 11:21, 22 August 2012

2012-08-22T11:21:19Z

2011-09-10T02:33:31Z

2011-09-10T02:19:44Z

2011-08-31T18:54:17Z

2011-07-19T12:38:20Z

2011-07-15T19:05:28Z

Protected "Main Page/microscopy I" ([edit=sysop] (indefinite) [move=sysop] (indefinite))

2011-01-17T21:02:05Z

48 revisions

2010-11-26T20:02:59Z

2010-11-26T20:00:39Z

2010-11-26T19:56:18Z

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 <h1>Required Components</h1>
 <ol>
-<li>[[Media:M1_Diatom.JPG|Diatom slide]]</li>
+<li>[[Media:Diatom_Slide.png|Diatom slide]]</li>
 <li>[[Media:Micrometer_Slide.JPG|Stage micrometer slide]]</li>
 <li>[[Media:M1_Baboon.JPG|Stained and unstained baboon eye slides]]</li>

@@ Line 321: / Line 321: @@
 when using the 40x objective.</li>
        <li>Press the ‘Live’ button and prepare to adjust the CCD exposure setting.</li>
-       <li>Adjust the CCD exposure setting so that you get bright image, trying to minimize
+       <li>Adjust the CCD exposure setting so that you get as bright an image as possible without saturating the CCD pixels. You can tell when you have a good exposure setting by observing the histogram in the lower portion of the μManager window.</li>
         <li>Click on ‘Full’ and you will remove any digital contrast adjustment from the live
 display. Set the exposure time so that the histogram in the graph window is

@@ Line 213: / Line 213: @@
 <li><h3>Working with immersion objectives</h3>
       <ol style="list-style-type:lower-latin">
-      <li>One of the most critical points of working with immersion objectives is to be very careful
+      <li>When working with immersion objectives be very careful with how much oil you use and clean the objective thoroughly when finished.</li>
       <li>Also be careful not to get any oil on the non-immersion objectives. If this does happen,
 remove the objective from the microscope and clean it with the lens cleaning solution.
-Remove one of the microscopes eyepieces and use it to examine the lens and ensure it
+Remove one of the microscope's eyepieces and use it to examine the lens and ensure it
 is clean. Use the lens to look at a specimen to confirm it is clean. If it is still dirty, repeat
 the cleaning procedure.</li>
       <li>Move the stage down and use the light from the condenser as a reference for where to
 place the drop on the specimen. You don’t need much immersion oil here, if you use
-too much it will be a waste and make it more difficult to clean the slide when we are
+too much it will be a waste and make it more difficult to clean the slide when you are
 finished.</li>
       <li> Carefully finish rotating the 100x or 40x objective in place and re-focus on the sample.

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 focal length and object size/position are known. When an object lies at a distance of 2f from the
 lens, the image is formed at -2f and the magnification factor is -1 (ie, the image is inverted). As
-the lens is moved closer to the object (decreasing dobj), the image distance and magnification
+the lens is moved closer to the object (decreasing d<sub>obj</sub>), the image distance and magnification
 increase.</p>
 <p>It is this image forming principle that is the basis for optical microscopes. The most basic form

@@ Line 4: / Line 4: @@
 <li>[[Media:Micrometer_Slide.JPG|Stage micrometer slide]]</li>
 <li>[[Media:M1_Baboon.JPG|Stained and unstained baboon eye slides]]</li>
-<li>Fresh onion</li>
+<li>[[Media:Slide_Tools.JPG|Fresh onion]]</li>
-<li>Knife and tweezers</li>
+<li>[[Media:Slide_Tools.JPG|Knife and tweezers]]</li>
-<li>Toothpick or popsicle sticks</li>
+<li>[[Media:Slide_Tools.JPG|Toothpick or popsicle sticks]]</li>
-<li>Blank slides and coverslips</li>
+<li>[[Media:Slide_Tools.JPG|Blank slides and coverslips]]</li>
-<li>Syringe of vaseline with pipet tip end</li>
+<li>[[Media:Slide_Tools.JPG|Syringe of vaseline with pipet tip end]]</li>
-<li>Distilled water</li>
+<li>[[Media:Slide_Tools.JPG|Distilled water]]</li>
 <li>[[Media:Immersion_Oil.JPG|Immersion oil]]</li>
 <li>[[Media:CCD.JPG|Nikon upright microscope and CCD camera]]</li>

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