Main Page/BPHS 4090/Choloplast Translocation

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Required Components

  1. Stock culture of Eremosphaera viridis
  2. Glass slides, cover slips and micropipette
  3. Nikon Optiphot Microscope
  4. Radiometric Probe (Model S471 Portable Optometer, UDT Instruments)
  5. Cool Snap CCD Camera and MicroManager software
  6. Blue and red band-pass filters

Objective

To observe the wavelength dependence of light on chloroplast translocation in the acidophile green algae Eremosphaera viridis as well as describing a mechanism behind this response.

Introduction

Electromagnetic radiation is the driving force for photosynthesis. Plants and other autotrophs convert the energy of light into chemical energy used for synthesizing carbohydrates and other organic compounds, which heterotrophic organisms use for energy and other nutritional requirements. Photosynthesis is a unique process performed by plants, algae, and some species of bacteria. It consists of a series of oxidation and reduction reactions; the basic chemical formula is shown below:

Ct eqn0.png


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